control non tumourigenic semi normal breast epithelial cell line mcf10a  (ATCC)

Journal: Frontiers in Pharmacology

Article Title: A novel scorpine-like peptide from the amazonian scorpion Brotheas amazonicus with cytolytic activity

doi: 10.3389/fphar.2025.1652614

Figure Lengend Snippet: Antitumor activity of Brotheas amazonicus scorpion crude venom. MCF10A, SKBR3, MCF7, and MDA-MB-231 cell lines (seeded at 1 × 10 5 cells/well) were treated and incubated with venom concentrations of 2, 10, 50, and 250 μg/mL for 24 h. Then, cell viability was assessed using the MTT assay. Data represent the mean ± SD of triplicate measurements from 4 independent experiments. NC - negative control (only culture medium). PC - positive control (30% DMSO). Statistical significance: group vs. NC, * p < 0.05.

Article Snippet: The breast tumor cell lines SKBr3, MCF-7, and MDA-MB-231, as well as the control breast epithelial cell line MCF10A, were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 (Roswell Park Memorial Institute) supplemented with 10% FBS and 1% penicillin/streptomycin at 37 C, 5% CO 2 .

Techniques: Activity Assay, Incubation, MTT Assay, Negative Control, Positive Control

Journal: Frontiers in Pharmacology

Article Title: A novel scorpine-like peptide from the amazonian scorpion Brotheas amazonicus with cytolytic activity

doi: 10.3389/fphar.2025.1652614

Figure Lengend Snippet: Antitumor activity of fractions from BamazV separated by molecular weight. Fractions were separated through an ultrafiltration process using a regenerated cellulose membrane (Amicon ® Ultra-15 Centrifugal Filter Ultracel ® 10K and 3K). MCF10A, SKBR3, MCF7, and MDA-MB-231 cell lines (seeded at 1 × 10 5 cells/well) were treated and incubated with BamazV fractions (<3 kDa, 3–10 kDa, and >10 kDa) at concentrations of 0.2, 1, 5, and 25 μg/mL for 24 h. Then, cell viability was assessed using the MTT assay. Data represent the mean ± SD of triplicate measurements from 3 independent experiments. NC - negative control (only culture medium). PC - positive control (30% DMSO). Statistical significance: group vs. NC, * p < 0.05.

Article Snippet: The breast tumor cell lines SKBr3, MCF-7, and MDA-MB-231, as well as the control breast epithelial cell line MCF10A, were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 (Roswell Park Memorial Institute) supplemented with 10% FBS and 1% penicillin/streptomycin at 37 C, 5% CO 2 .

Techniques: Activity Assay, Molecular Weight, Membrane, Incubation, MTT Assay, Negative Control, Positive Control

Journal: Frontiers in Pharmacology

Article Title: A novel scorpine-like peptide from the amazonian scorpion Brotheas amazonicus with cytolytic activity

doi: 10.3389/fphar.2025.1652614

Figure Lengend Snippet: Antitumor activity of FPLC peaks from Bamaz>10 fraction. Peaks P31, P59, P75 and P81 were tested for antitumor effects in breast cancer cell lines. MCF10A, SKBR3, MCF7, and MDA-MB-231 cell lines (seeded at 1 × 10 5 cells/well) were treated and incubated with the indicated doses (see graphs) for 24 h. Then, cell viability was assessed using the MTT assay. Data represent the mean ± SD of triplicate measurements from 4 independent experiments. NC - negative control (only culture medium). PC - positive control (30% DMSO). Statistical significance: group vs. NC, * p < 0.05.

Article Snippet: The breast tumor cell lines SKBr3, MCF-7, and MDA-MB-231, as well as the control breast epithelial cell line MCF10A, were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 (Roswell Park Memorial Institute) supplemented with 10% FBS and 1% penicillin/streptomycin at 37 C, 5% CO 2 .

Techniques: Activity Assay, Incubation, MTT Assay, Negative Control, Positive Control

Journal: Frontiers in Pharmacology

Article Title: A novel scorpine-like peptide from the amazonian scorpion Brotheas amazonicus with cytolytic activity

doi: 10.3389/fphar.2025.1652614

Figure Lengend Snippet: Viability assay and type of cell death after treatment with the scorpine-like peptide. MCF10A, SKBR3, MCF7, and MDA-MB-231 cell lines (seeded at 1 × 10 5 cells/well) were treated and incubated with BamazScplp1 (50 μg/mL) or controls (NC: culture medium; PC: 30% DMSO) for 24 h. From left to right, cell viability was assessed using the MTT assay. Representative flow cytometry dot plots (NC vs. BamazScplp1-treated cells) and quantification of apoptotic and necrotic cell death after treatment. Data represent the mean ± SD of triplicate measurements from 1 experiment. NC - negative control (only culture medium). Statistical significance: group vs. NC, * p < 0.05.

Article Snippet: The breast tumor cell lines SKBr3, MCF-7, and MDA-MB-231, as well as the control breast epithelial cell line MCF10A, were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 (Roswell Park Memorial Institute) supplemented with 10% FBS and 1% penicillin/streptomycin at 37 C, 5% CO 2 .

Techniques: Viability Assay, Incubation, MTT Assay, Flow Cytometry, Negative Control

Journal: Molecular medicine reports

Article Title: Isorhamnetin inhibits cell proliferation and induces apoptosis in breast cancer via Akt and mitogen‑activated protein kinase kinase signaling pathways.

doi: 10.3892/mmr.2015.4269

Figure Lengend Snippet: Figure 1. Isorhamnetin inhibits proliferation and induces apoptosis of breast cancer cells. (A) The cells were treated with various concentrations of isorhamnetin for 72 h, cell proliferation was determined using a Cell Counting kit-8 (CCK‑8) assay and the IC50 was calculated. *P<0.05, IC50 of isorhamnetin in various cancer cells as compared with the MCF10A cells. (B) Isorhamnetin induced apoptosis of the MCF7 and MDA‑MB‑468 cells following a 48‑h treatment. The upper right quandrant shows early apoptotic cells, the lower right the late apoptotic cells, the upper left quadrant indicates cell debris and the lower left the viable cells. IC50, half maximal inhibitory concentration; PI, propidium iodide.

Article Snippet: MCF7, T47D, BT474, BT-549, MDA-MB-231 and MDA-MB-468 breast cancer cell lines, as well as a MCF10A normal breast epithelial cell line (control) were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Cell Counting, CCK-8 Assay, Concentration Assay